Tag Archives for staining

I’ve worked out a method for doing immunohistochemistry (IHC) on formalin-fixed paraffin embedded (FFPE) sections of bone.

Bone is very autofluorescent, so most dyes that excite in the visible range also excite substances present in bone itself, making it hard to find the specific signal generated by the dye-conjugated antibody. However, I noticed when using DAPI, an … Continue reading

31. August 2007 by Mr. Gunn
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